[Retracted] CircAGFG1 Promotes Osteosarcoma Progression and Stemness by Competing with miR-302a-3p to Upregulate the Expression of LATS2

Figure 5

The correlation of circAGFG1, LATS2, and miR-302a-3p in osteosarcoma cell lines and xenograft tumors. (a) Detection of miR-302a-3p transfection efficiency in U2OS and HOS cells. (b) Detection of LATS2 transfection efficiency in U2OS and HOS cells. (c) U2OS cell proliferation test showed that the cell proliferation ability was significantly reduced in the cells mock-transfected with LATS2-siRNA or miR-302a-3p. The overexpression of circAGFG1 partially reduced the effect of LATS2-siRNA or miR-302a-3p in U2OS cells. (d) The results of cell proliferation assay in HOS cells are similar. (e) U2OS cell clone formation test showed that, in the cells mock-transfected with LATS2-siRNA or miR-302a-3p, the cell clone formation ability was significantly reduced. The overexpression of circAGFG1 partially reduced the effect of LATS2-siRNA or miR-302a-3p in U2OS cells. (f) The results of cell cloning assay in HOS cells are similar. (g) U2OS cell Transwell test showed that in the cells mock-transfected with LATS2-siRNA or miR-302a-3p, the cell migration ability was significantly reduced. The overexpression of circAGFG1 partially reduced the effect of LATS2-siRNA or miR-302a-3p in U2OS cells. (h) The results of cell Transwell assay in HOS cells are similar. .

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