Shipi Shugan Decoction Protected against Sequela of Pelvic Inflammatory Disease via Inhibiting SIRT1/NLRP3 Signaling Pathway in Pelvic Inflammatory Disease Rats

Figure 1

SSD dose-dependently attenuated inflammation of uterine and fallopian tube in SPID rats. 35 female rats were used for these experiments: Sham group (n = 8), SPID group (n = 6), high-dose SSD group (n = 8), low-dose SSD group (n = 6), and Fuke Qianjin Capsules (FQC) group (n = 7). After the SPID model was established, the rats in the high-dose SSD group and low-dose SSD group were intragastrically administered with 6.64 g/kg • day and 1.66 g/kg • day SSD, respectively. The rats in the FQC group were intragastrically administered with 0.22 g/kg • day after the suspension of FQC. The rats in the sham group and SPID group were given the same amount of normal saline. The contents of TNF-α (a) IL-1β (b) IL-6 (c) and IL-18 (d) in serum samples of rats in each group were tested by enzyme-linked immunosorbent assay (ELISA). (e) The numbers of WBCs and lymphocytes were counted under a microscope. (f) H&E stain was used to observe the pathological changes in the fallopian tube and uterine tissues. The magnification is 200× and 400×. Yellow arrow: vasodilation and hyperemia; green arrow: degeneration and necrosis of epithelial cells; blue arrow: lymphocyte; black arrow: endometrial lamina propria edema. H&E: hematoxylin and eosin; WBCs: white blood cells; TNF: tumor necrosis factor; IL: interleukin; SPID: sequela of pelvic inflammatory disease; SSD: Shipi Shugan Decoction; FQC: Fuke Qianjin Capsules. (versus sham), (versus sham), (versus sham), (versus SPID), (versus SPID), and (versus SPID). Data represent the Mean ± SEM.

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