Shipi Shugan Decoction Protected against Sequela of Pelvic Inflammatory Disease via Inhibiting SIRT1/NLRP3 Signaling Pathway in Pelvic Inflammatory Disease Rats

Figure 4

SSD against inflammation and pathological damage of upper genital tract by inhibiting SIRT1/NLRP3 signaling. 33 rats were used for these experiments: sham group (n = 8), SPID group (n = 6), SSD group (n = 6), SIRT1 inhibitor (EX527) group (n = 6), and SSD + EX527 group (n = 7). After the SPID model was established, the rats in the EX527 group were intragastrically administered with a 5 mg/kg/day SIRT1 inhibitor (Sigma, Munich, Germany; No. E7034). The rats in SSD + EX527 group were intragastrically administered with 5 mg/kg/day and 6.64 g/kg • day SSD. The rats in the sham group and SPID group were given the same amount of normal saline. The content of TNF-α (a) IL-1β (b) IL-6 (c) and IL-18 (d) in serum samples of rats in each group was detected by enzyme-linked immunosorbent assay (ELISA). (e) The numbers of WBCs and lymphocytes were counted under a microscope. (f) H&E stain was used to observe the pathological changes in the fallopian tube and uterine tissues. The magnification is 200× and 400×. Yellow arrow: vasodilation and hyperemia; blue arrow: lymphocyte; black arrow: endometrial lamina propria edema; H&E: hematoxylin and eosin; WBCs: white blood cells; TNF: tumor necrosis factor; IL: interleukin; SPID: sequela of pelvic inflammatory disease; SSD: Shipi Shugan Decoction; FQC: Fuke Qianjin Capsules. (versus sham), (versus sham), (versus SPID), (versus SPID), (versus SSD), and (versus SSD). Data represent the Mean ± SEM.

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