Modified Yuejuwan Inhibited Cholesterol Accumulation and Inflammation in THP-1 Macrophage-Derived Foam Cells by Inhibiting the Activity of the TRIM37/TRAF2/NF-κB Pathway

Figure 3

Overexpression and knockdown of TRIM37 and its silencing promoted cholesterol efflux in foam cells. (a) The relative mRNA levels and relative protein contents of TRIM37 in different transfected cells as indicated. vs. oeNC. (b) TRIM37 silencing inhibiting the uptake of oxLDL in foam cells. Magnification: 200x. TC, FC, and CE contents downregulated in siTRIM37-1 or siTRIM37-2 transfected cells. vs. siNC. (c) Knockdown of TRIM37 inhibiting the secretion of IL-1β and TNF-α in foam cells. vs. siNC. (d) Western blot used to quantify the protein content of SR-B1, ABCA1, ABCG1, NF-κB (cytoplasm), and NF-κB (nucleus) in cells as indicated above. vs. siNC.

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