Ruscogenin Ameliorated Sjögren’s Syndrome by Inhibiting NLRP3 Inflammasome Activation

Figure 2

Ruscogenin promoted the AQP5 and AQP4 expression while suppressed inflammation-related factors expression in submandibular gland tissues of NOD/ShiLtJ mice. (a and b) Representative images of protein bands (a) and relative protein expression of AQP5 and AQP4 (b) in submandibular gland tissues was tested by western blot after treatment of vehicle and Ruscogenin. GAPDH is a loading control. (c and d) Representative images of protein bands (c) and relative protein expression of P2X7R, NLRP3, caspase 1, and IL-1β (d) in submandibular gland tissues was assessed by western blot after treatment of vehicle and Ruscogenin. GAPDH is a loading control. , , vs. vehicle group. All experiments were repeated independently at least three times. Data were performed as the means ± standard deviation. AQP: aquaporin; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; P2X7R: purinergic receptor P2X ligand-gated ion channel 7; NLRP3: nucleotide binding oligomerization domain-like receptor 3; IL: interleukin.

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