Mechanism of Sishen-Pill-Regulated Special Memory T and mTfh Cell via Involving JAK/STAT5 Pathway in Colitis Mice

Figure 3

SSP regulates CD4⁺ TSM cells and their subsets in colitis mice. After being stained with different fluorescent antibodies, cells were sequentially gated on living lymphocytes, CD4⁺, CCR7⁺, GL7⁺, and CD62L⁺. CD4⁺CCR7⁺GL7⁺, CD4⁺CCR7⁺CD62L⁺, and CD4⁺CCR7⁺CD62L⁺GL7⁺ TSM cells were analyzed by flow cytometry. (a) Total lymphocytes in peripheral blood. (b) CD45RA⁺cells. (c) The proportion of CCR7⁺ labeled CD4⁺ TSM cells. (d) Relative percentages of CD4⁺CCR7⁺GL7⁺ cells in UC mice pre- and post-treatment. A: norl group, B: DSS, C: DSS + SSP, D: DSS + 5-ASA, and E: CD4⁺CCR7⁺GL7⁺ cells level. (e) Relative percentages of CD4⁺CCR7⁺CD62L⁺ cells in UC mice pre- and post-treatment. A: nor group, B: DSS, C: DSS + SSP, D: DSS + 5-ASA, and E: CD4⁺CCR7⁺CD62L⁺ cells level. (f) Relative percentages of CD4⁺CCR7⁺CD62L⁺GL7⁺ cells in UC mice pre- and post-treatment. A: nor group, B: DSS, C: DSS + SSP, D: DSS + 5-ASA, and E: CD4⁺CCR7⁺CD62L⁺GL7⁺ cells level. Data are presented as mean ± SEM (n = 10). and versus nor group and and versus DSS group.