Mechanism of Sishen-Pill-Regulated Special Memory T and mTfh Cell via Involving JAK/STAT5 Pathway in Colitis Mice

Figure 4

SSP regulates CD4⁺ mTfh cells and their subsets in colitis mice. After being stained with different fluorescent antibodies, the cells were sequentially gated on living lymphocytes, CD4⁺, CCR7⁺, and CXCR5⁺. The proportion of CD4⁺CCR7⁺CXCR5⁺mTfh cells was determined. Subsequently, CD4⁺mTfh cells were gated on GL7 and CD62L, and the proportion of GL7⁺CD4⁺mTfh cells and CD62⁺CD4⁺mTfh cells in lymphocytes was analyzed. (a) Total lymphocytes in peripheral blood. (b) CD45RA⁺ cells. (c) The proportion of CCR7⁺ labeled CD4⁺ T cells. (d) Relative percentages of GL7⁺CD4⁺mTfh cells in UC mice pre- and post-treatment. A: nor group, B: DSS, C: DSS + SSP, D: DSS+5-ASA, and E: GL7⁺CD4⁺cells level. (e) Relative percentages of CD62⁺CD4⁺mTfh cells in UC. A: nor group, B: DSS, C: DSS + SSP, D: DSS + 5-ASA, and E: CD62⁺CD4⁺cells level. (f) Relative percentages of total CD4⁺mTfh cells in UC. A: nor group, B: DSS, C: DSS + SSP, D: DSS + 5-ASA, and E: CD4⁺CCR7⁺CXCR5⁺cells level. Data are presented as mean ± SEM (n = 10). and versus nor group and and versus DSS group.