Protective Effects of Interleukin-37 Expression against Acetaminophen-Induced Hepatotoxicity in Mice

Figure 1

Detection the expression of exogenous IL-37. (a) IL-37 mRNA levels in mouse liver tissues analyzed by RT-PCR at 0, 6, 12, 24, 48, 72, and 96 h after hydrodynamic procedures. Data are expressed as mean ± SD of animals analyzed at each time point (n = 5). (b) IL-37 protein levels in liver homogenate analyzed by ELISA at each similar time point. Data are expressed as mean ± SD of animals analyzed at each time point (n = 5). IL-37 mRNA and protein levels were normalized with β-actin expression. IL-37 protein expression was detected in mouse livers 24 h after injection of empty pcDNA3.1 (c) and pcDNA3.1-IL-37 (d) plasmids using immunohistochemical staining.

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