Protective Effects of Interleukin-37 Expression against Acetaminophen-Induced Hepatotoxicity in Mice

Figure 4

Influence of IL-37 on expression of inflammatory cytokines mRNAs and NF-κB p65 proteins in APAP-induced liver injury. Mice were pretreated with pcDNA3.1-IL-37 at 24 h before APAP injection. Liver tissue samples were collected at 24 h after APAP administration for the detection of TNF-α (a), IL-6 (b), and IL-17 (c) by RT-PCR. Liver tissues were collected at 24 h after APAP administration for Western blot detections (d) and the corresponding densitometry analysis (e) of NF-κB p65. β-Actin was used as an internal control. Data are expressed as mean ± SD (n = 6–8 per group, , , and vs. the pcDNA3.1/APAP group).

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