Shikonin Mediates Apoptosis through G Protein-Coupled Estrogen Receptor of Ovarian Cancer Cells

Figure 1

Effects of different concentrations of SK on the proliferation of SKOV3 and A2780 cells. (a) 2D and 3D chemical structure of SK. (b) Cell viability of SKOV3 cells treated with different concentrations of SK for different times. (c) LDH production was measured using an LDH release assay kit in SKOV3 cells treated with 5 and 10 μM SK for 24 h. (d) Plate clone formation experiments including SKOV3 cells with SK at the indicated concentrations (5 and 10 μM) for two weeks. The number of clones was counted. (e) Cell viability of A2780 cells treated with different concentrations of SK for different times. (f) LDH production was measured using an LDH release assay kit in A2780 cells treated with 0.5 and 1.0 μM SK for 24 h. (g) A2780 cells with SK at the indicated concentrations (0.5 and 1.0 μM) for two weeks. The number of clones was counted. Data was represented as means ± SEM. , vs. control group were considered as statistically significantly.

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