Shikonin Mediates Apoptosis through G Protein-Coupled Estrogen Receptor of Ovarian Cancer Cells

Figure 2

. Effects of SK on the apoptosis of SKOV3 and A2780 cells. (a) Representative dot‑plots from cytometrically illustrating apoptotic status in SKOV3 cells with 5 and 10 μM SK for 24 h. (b) Statistical analysis of apoptotic (percentage of cells in Q2 and Q3) SKOV3 cells. (c) The expression and quantitatively analysis of Bax and Bcl-2 proteins in SKOV3 cells treated with 5 and 10 μM SK for 24 h. (d) Representative dot‑plots from cytometrically illustrating apoptotic status in A2780 cells with 0.5 and 1.0 μM SK for 24 h. (e) Statistical analysis of apoptotic (percentage of cells in Q2 and Q3) A2780 cells. (f) The expression and quantitatively analysis of Bax and Bcl-2 proteins in A2780 cells treated with 0.5 and 1.0 μM SK for 24 h. Images are representative of three independent experiments. Data are mean ± S.E.M. from three independent experiments. , vs. control group were considered as statistically significantly.

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