Shikonin Mediates Apoptosis through G Protein-Coupled Estrogen Receptor of Ovarian Cancer Cells

Figure 6

SK inhibits SKOV3 and A2780 cells viability and promotes apoptosis via the GPER mediated pathway. (a) Cell viability of SKOV3 cells treated with 8.6 μM SK and 10 nM G1 or 20 nM G15 for 24 h. (b) Representative dot‑plots from cytometrically illustrating apoptotic status in SKOV3 cells treated with 8.6 μM SK and 10 nM G1 or 20 nM G15 for 24 h. (c) Statistical analysis of apoptotic (percentage of cells in Q2 and Q3) SKOV3 cells. (d) The expression and quantitatively analysis of Bax and Bcl-2 proteins in SKOV3 cells treated with 8.6 μM SK and 10 nM G1 or 20 nM G15 for 24 h. (e) Cell viability of A2780 cells treated with 0.56 μM SK and 10 nM G1 or 20 nM G15 for 24 h. (f) Representative dot‑plots from cytometrically illustrating apoptotic status in A2780 cells treated with 0.56 μM SK and 10 nM G1 or 20 nM G15 for 24 h. (g) Statistical analysis of apoptotic (percentage of cells in Q2 and Q3) A2780 cells. (h) The expression and quantitative analysis of Bax and Bcl-2 proteins in A2780 cells treated with 0.56 μM SK and 10 nM G1 or 20 nM G15 for 24 h. The results are means of three independent replicates ± S.D. or vs. control group, ^△△ or ^△ vs. SK treating group were considered as statistically significant.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)