The Mitigatory Effect of Shen-Qi Compound on the Diabetic Thoracic Aortic Complications through Inhibiting the Inflammatory Microenvironment by miR-223-3p/RBP-J/IRF8 Axis

Figure 5

SQC regulated the expression of helper T cells in rats with diabetic thoracic aortic complications. (a–d) The relative mRNA expressions of T-bet (a), GATA3 (b), ROR-γt (c), and Foxp3 (d) were detected by qRT-PCR. The gene expressions were normalized by β-actin. (e–i) The relative protein expressions of T-bet (a), GATA3 (b), ROR-γt (c), and Foxp3 (d) were examined by western blot. The gene expressions were normalized by β-actin. (j–p) The concentrations of IL-6 (j), TNF-α (k), IFN-γ (l), IL-4 (m), TGF-β1 (n), IL-17A (o), and IL-10 (p) were measured by ELISA. vs. Control group. ^#vs. Model group. All assays were performed six times.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)

(k)

(l)

(m)

(n)

(o)

(p)