Paeoniflorin Enhances the Sensitivity of ER-Positive Breast Cancer Cells to Tamoxifen through Promoting Sirtuin 4

Figure 4

Paeoniflorin inhibits the proliferation of ER-positive breast cancer cells through SIRT4-mediated mitochondrial metabolism. (a) T47D and MCF-7 cells were treated with or without 60 μM PF for 48 hours, and we harvested the cells to detect the expression of SIRT4 using western blot, and the representative proteins brand were showed in the right. (b) 48 hours after transfecting with specific small interfering RNA for SIRT4 (si-SIRT4), SIRT4 protein expression was analyzed by western blot in T47D and MCF-7 cells, and the representative proteins brand were showed in the right. (c) 48 hours after transfecting with si-SIRT4, T47D and MCF-7 cells were treated with or without 60 μM PF for 48 hours, and the cell viability was determined using the CCK8 assay kit. 48 hours after transfecting with si-SIRT4, T47D, and MCF-7 cells were treated with or without 60 μM PF for 48 hours, and we harvested the cells to detect the extracellular acidification rate (ECAR) (d) and oxygen consumption rate (OCR) (e) using a Seahorse XF24 extracellular flux analyzer. Data were expressed as mean ± SD with 3 independent repetitions. ^ns, , , and between two groups.

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