Taohong Siwu Decoction Promotes Osteo-Angiogenesis in Fractures by Regulating the HIF-1α Signaling Pathway

Figure 3

TSD promotes the proliferation, angiogenesis, and migration of the RAECs. (a) Immunofluorescence analysis for primary RAECs identification (400×); scale bar, 25 μm. (b) Representative images and quantification statistics of HE-stained RAECs (100× and 400×); scale bar, 100 μm and 25 μm. (c) MTT detection of RAECs proliferation. (d) Representative images of tube forming experiment (100×); scale bar, 100 μm. (e) Quantitative statistics of (D). (f) Representative images of transwell migration experiment (100×); scale bar, 100 μm. (g) The OD value of migrated cells from the migration experiment. ns: no statistical significance; vs. the control serum group. ^### vs. the TSD group. TSD: Taohong Siwu decoction; RAECs: rat aortic endothelial cells; HE: hematoxylin-eosin; MTT: methyl tetrazolium; and OD: optical density.

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