Bellidifolin Inhibits SRY-Related High Mobility Group-Box Gene 9 to Block TGF-β Signalling Activation to Ameliorate Myocardial Fibrosis

Figure 4

SOX9 knockdown suppressed TGF-β1/Samd3 pathway activation. (a, c, e) Western blot and qRT-PCR evaluated siRNA transfection efficiency, and bar graphs presented fold changes of SOX9 expression. GAPDH was used as a loading control (n = 3). (b, d, f) Western blot and qRT-PCR analysed the effect of SOX9-Homo-905 siRNA on SOX9 administrated by TGF-β1. (g, h) The effect of knockdown of SOX9 on the expression of TGF-β1 and Smad3 and the level of Smad3 phosphorylation in the presence of TGF-β1 were detected by western blot. (i, j) The fold changes of TGF-β1 and P-Smad3/Smad were presented by bar graphs. GAPDH was used as a loading control (n = 3). Data were presented as mean ± SEM. and vs. Control + NC; ^# vs. TGF-β1 + NC.

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