Anti-inflammatory Mechanism of Action of Benzoylmesaconine in Lipopolysaccharide-Stimulated RAW264.7 Cells

Figure 3

Effect of BMA on TNF-α and IL-1β levels in LPS-activated RAW264.7 cells. ((a)–(c)) The levels of TNF-α, IL-1β, and IL-6 were determined by ELISA after treatment with BMA (40, 80, or 160 μM) for 1 h and stimulation with LPS (1 μg/mL) for another 24 h in LPS-activated RAW264.7 cells. ((d)-(f)) The mRNA levels of TNF-α, IL-1β, and IL-6 were determined by real-time PCR. Data are expressed as mean ± SD from three independent experiments. vs. control group, and , vs. LPS group.

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