Anti-inflammatory Mechanism of Action of Benzoylmesaconine in Lipopolysaccharide-Stimulated RAW264.7 Cells

Figure 4

Effect of BMA on the production of PGE₂, NO, and ROS in LPS-activated RAW264.7 cells. (a) Cells were treated with BMA (40, 80, or 160 μM) for 1 h and stimulated with LPS (1 μg/mL) for 24 h The level of ROS was determined using fluorescence microscopy. (b) Cells were treated with BMA (40, 80, or 160 μM) for 1 h and stimulated with LPS (1 μg/mL) for 24 h The level of PGE₂ production was determined using ELISA. (c) SP600125, TPCK, SB202190, or U0126 was administered to macrophages separately with or without BMA (160 μM) for 1 h, and then cells were stimulated with LPS (1 μg/mL) for 24 h The absorbance at 540 nm was measured using a microplate reader. Data are expressed as mean ± SD from three independent experiments. vs. control group, and , vs. LPS group.

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