Anti-inflammatory Mechanism of Action of Benzoylmesaconine in Lipopolysaccharide-Stimulated RAW264.7 Cells

Figure 5

Effect of BMA on the protein expression of COX-2 and iNOS in LPS-activated RAW264.7 cells. ((a)-(c)) Cells were treated with BMA (40, 80, or 160 μM) for 1 h and then stimulated with LPS (1 μg/mL) for 24 h. The level of protein expression was analyzed by western blot, and β-actin was used as a loading control. (d) and (e) The mRNA levels of COX-2 and iNOS were determined by real-time PCR. Data are expressed as mean ± SD from three independent experiments. vs. control group, and , vs. LPS group.

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