Antrodia salmonea Extracts Regulate p53-AR Signaling and Apoptosis in Human Prostate Cancer LNCaP Cells

Figure 1

AS induces apoptosis of LNCaP cells. (a) LNCaP cells were seeded in 12-well plates and then treated with 6.25, 12.5, 25, 50, and 100 μg/ml AS for 24 hours followed by trypan blue assay. Data were presented as the mean ± S.E.M. (Standard error of the mean) with three independent experiments (n = 3) and paired Student’s t-test was used to calculate the -value. Statistical significance was marked as , , and . (b) LNCaP cells were seeded in 12 well plates and then treated with AS (0, 25, 50, and 100 μg/ml) for 24 hours. Protein expression of apoptotic markers was evaluated by western blot with the specific primary antibodies anticleaved PARP, anti-PARP, anticleaved caspase 3, anticleaved caspase 8, anticleaved caspase 9, anti-GAPDH, and antiactin. GAPDH and actin were served as internal controls.

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