Antrodia salmonea Extracts Regulate p53-AR Signaling and Apoptosis in Human Prostate Cancer LNCaP Cells

Figure 3

AS increases the protein level of p53 and reduces its protein degradation. (a) LNCaP cells were seeded in 12-well plates and followed with the treatment of AS with a series of concentrations (0, 6.25, 12.5, 25, 50, and 100 μg/ml) for 24 hours. Western blot analysis was performed to identify the protein levels of p53 after AS treatment, and the protein level of GAPDH was served as an internal control. (b) The quantitative results of (a) were shown. (c) LNCaP cells were seeded in 12-well plates and then treated with AS (0, 25, and 50 μg/ml) for 24 hours. After that, the cells were treated with 10 μg/ml cycloheximide for 0, 3, and 5 hours before protein extraction. Western blot analysis was then performed to evaluate the protein level of p53 with specific p53 antibody. (d) The quantitative data of the p53 degradation curves were shown and the original p53 protein levels of all groups were adjusted as 1. Data were presented as the mean ± S.E.M. (Standard error of the mean) with three independent experiments (n = 3) and the paired Student’s t-test was used to calculate the -value compared to group = 0. Significance was marked as , , and .

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