Antrodia salmonea Extracts Regulate p53-AR Signaling and Apoptosis in Human Prostate Cancer LNCaP Cells

Figure 4

AS reduces expression and phosphorylation of AR, and causes nuclear localization of p53. (a) LNCaP cells were seeded in 12-well plates and then treated with AS (0, 6.25, 12.5, 25, 50, and 100 μg/ml) for 24 hours. Western blot analysis was performed to evaluate the protein levels of AR phosphorylation at Ser-81 and total AR in LNCaP cells. (b) The quantitative results of (a) were shown, where actin was used as an internal control. (c) LNCaP cells were treated with AS (0, 25, and 100 μg/ml) for 24 hours. Protein extraction was performed by nucleus/cytosol cell fractionation. Protein levels were detected by western blotting with specific antibodies targeting AR, p-s81-AR, p53, p21, and PARP. Actin protein was served as an internal control. PARP and tubulin were respectively served as nuclear and cytosolic markers. (d) The quantitative results of (c) were shown. Data were presented as the mean ± S.E.M. (standard error of the mean) with three independent experiments (n = 3) and the paired Student’s t-test was used to calculate the -value compared to group = 0. Significance was marked as , , and .

(a)

(b)

(c)

(d)