Comparison of Protective Effects of Shenmai Injections Produced by Medicinal Materials from Different Origins on Cardiomyocytes

Figure 1

Protective effects of Shenmai injections on cardiomyocyte injury induced by hypoxia-reoxygenation. (a) H9c2 cells were cultured with mediums containing different concentrations of glucose (HG, high glucose, mediums with 4.5 g/L glucose; LG, low glucose, mediums with 1 g/L glucose; NG, no glucose, mediums with 0 g/L glucose) in a humidified incubator with 5% CO₂ and 95% N₂ for 24 h after which cells were cultured in 5% CO₂ and 95% air for another 3 h. Then, cell viability was detected using CCK-8 assays (HR, hypoxia-reoxygenation). (b–d) Cytoprotective effects of 1% ((V):V) Shenmai injections in different models. (e, f) ROS levels of a, b. , , vs corresponding control groups, Student’s t-test. vs SM5 group, Student’s t-test.

(a)

(b)

(c)

(d)

(e)

(f)