Marsdenia tenacissima Extract Induces Autophagy and Apoptosis of Hepatocellular Cells via MIF/mToR Signaling

Figure 6

MTE induced the autophagy of HCC cells by inhibiting the Akt/mTOR pathway via MIF. (a) LC3 I, LC3 II, caspase3, cleaved caspase3, MIF, CD74, Beclin-1, and β-actin protein levels in MTE (17.5 mg/mL, 35 mg/mL, and 70 mg/mL)-treated MHCC-97H cells were examined by WB assay. (b) Akt, mTOR, caspase 3, and LC 3 mRNA levels in MTE (17.5 mg/mL, 35 mg/mL, and 70 mg/mL)-treated MHCC-97H cells were detected by qRT-PCR assay. (c) The mRNA levels for Akt, mTOR, caspase 3, and LC 3 genes in MTE (25 mg/mL, 50 mg/mL, and 100 mg/mL)-treated HepG2 cells were detected by qRT-PCR assay. (d) The mTOR, Akt, and GAPDH protein levels in MTE (17.5 mg/mL, 35 mg/mL, and 70 mg/mL)-treated MHCC-97H cells were detected by WB assay. (e) The mTOR, Akt, and GAPDH protein levels in MTE (17.5 mg/mL, 35 mg/mL, and 70 mg/mL)-treated HepG2 cells were detected by WB assay. n = 3.

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