Lucidumol A, Purified Directly from Ganoderma lucidum, Exhibits Anticancer Effect and Cellular Inflammatory Response in Colorectal Cancer

Figure 4

Lucidumol A attenuates LPS-induced proinflammatory responses. (a) The cytotoxic effect of LPS and Lucidumol A in RAW264.7 cells. (b) Nitric oxide assay showing decreased nitric oxide production in RAW264.7 cells in response to Lucidumol A and LPS treatment. (c–e) The suppressed expression of COX-2 and iNOS in response to Lucidumol A. The cells were exposed to LPS (0.5 μg/ml) in the presence of various concentrations of Lucidumol A as indicated in each figure. Subsequently, the mRNA levels of COX-2 and iNOS genes were analyzed by a standard qRT-PCR analysis (c, d) and protein expression by Western blot analysis using anti-COX-2 and iNOS antibodies. The Western blot analysis was performed with three independent experiments and the summarized values after normalized by GAPDH were shown on the right two panels (e). All the expressions shown in this figure were performed in three independent experiments (n = 3). Bars mean ± S.D. and the value were obtained by Student’s t-test (n = 3, ; ).

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