Astragali Radix-Coptis Rhizoma Herb Pair Attenuates Atherosclerosis in ApoE-/- Mice by Regulating the M1/M2 and Th1/Th2 Immune Balance and Activating the STAT6 Signaling Pathway

Figure 4

The ARCR herb pair regulated the M1/M2 and Th1/Th2 balance. (a) The expression of F4/80 + CD11c+ and F4/80 + CD206+ cells was examined using flow cytometry. (b–d) Percentages of M1 and M2 macrophages and M1/M2 macrophages. (e) The expression of CD4 + CD25 + T-bet+ and CD4 + CD25 + GATA-3+ cells was examined. (f–h) Percentages of Th1 and Th2 cells and Th1/Th2. (i) Representative Western blot of iNOS (M1 marker) and Arg-1 (M2 marker) in aortas. (j-k) iNOS and Arg-1 expression relative to the β-actin level. (l) Representative Western blot of T-bet (Th1 marker) and GATA-3 (Th2 marker) in aortas. (m-n) T-bet and GATA-3 expression relative to the β-actin level. The data are presented as the means ± SD, n = 4. , vs. the control group; , vs. the model group; , vs. ARCR group; vs. Simva group.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)

(k)

(l)

(m)

(n)