The 5-HT and PLC Signaling Pathways Regulate the Secretion of IL-1β, TNF-α and BDNF from NG2 Cells

Figure 5

Effects of the specific activator m-3M3FBS and the specific inhibitor U-73122 of PLC on the secretion of IL-1β, TNF-α and BDNF in NG2 cells. (a-d) Real-time PCR was used to determine the expression of PLC, IL-1β, TNF-α and BDNF mRNA. (d-e) Western blotting was used to determine the expression of PLC, IL-1β, TNF-α and BDNF. GAPDH is shown as an internal control. (Data are expressed as the means +/− s.d. n = 3 for each group. , . s.d.: standard deviation).

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