The 5-HT and PLC Signaling Pathways Regulate the Secretion of IL-1β, TNF-α and BDNF from NG2 Cells

Table 1

Primer sequences used in this study.

Primer name		Sequences (5ʹ-3ʹ)	Product size (bp)
5-HT1AR	Forward	AAGGACCACGGCTACACCATCTAC	108
	Reverse	CTGACAGTCTTGCGGATTCGGAAG
5-HT2AR	Forward	CTTCCAACGGTCCATCCACA	132
	Reverse	GGGCACCACATTACAACAAACAG
5-HT3R	Forward	ACATTTCCCTGTGGCGAACA	189
	Reverse	CAGTGGTTTCCCATGGCTGAG
5-HT5R	Forward	CGCTGTGCTCCTGGGATAT	78
	Reverse	CCTGTTGAACGCCGTGTAGAT
5-HT6R	Forward	GCACGAACTGGGCAAAGCT	86
	Reverse	GGACGCCACGAGGACAAAA
5-HT7R	Forward	TTCTGTCGGTCTGGCTGCTCTC	130
	Reverse	CCGCAGTGGAGTAGATCGTGTAG
IL-1β	Forward	TGACAGGCAACCACTTACC	123
	Reverse	CCCATACACACGGACAACT
TNF-α	Forward	GAAACAGTCTGCGAGGTGTG	158
	Reverse	TTCTTCTTGCAGCCACACAC
BDNF	Forward	TCTACGAGACCAAGTGTAATCCCAT	166
	Reverse	GAAGTGTCTAT CCTTATGAACCGC
PLCβ1	Forward	CTGAGGGCTCACGCAAGAA	102
	Reverse	GCAGCACGGTATAGGTGAA
GAPDH	Forward	ACAGCAACAGGGTGGTGGAC	98
	Reverse	TTTGAGGGTGCAGCGAACTTT