Mechanistic Research into the Effects of the Jianpi Xiaozhi Formula on Liver Injury in Diabetic Rats

Figure 2

Effects of Jianpi Xiaozhi Formula (JPXZF) on mRNA and protein expression associated with endoplasmic reticulum stress (ERS) in the rat models of type 2 diabetes mellitus (T2DM) with nonalcoholic fatty liver disease (NAFLD) induced by a high-sugar and high-fat diet combined with streptozotocin (STZ) treatment. Normal control (NC group): healthy untreated rats; diabetes mellitus (DM) group: rat models of DM; DM + JL group: diabetic rats treated with low-dosage Jianpi Xiaozhi formula (JPXZF) (4.5 g/kg/d); DM + JM group: diabetic rats treated with an intermediate dosage of JPXZF (9 g/kg/d); DM + JH group: diabetic rats treated with high-dosage JPXZF (18 g/kg/d); DM + PBA group: diabetic rats treated with 4-phenylbutyric acid (PBA; 2.5 mg/kg/d). (a) The mRNA expression levels of glucose-regulated protein 78/immunoglobulin-heavy-chain-binding protein (GRP78) and activating transcription factor 6 (ATF6) were assessed by quantitative real-time polymerase chain reaction; (b, c) GRP78 and ATF6 protein expression was determined by Western blotting (WB). The data are expressed as the means ± standard deviation. versus the NC group; versus the DM group; versus the DM + JL group.

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