Mechanistic Research into the Effects of the Jianpi Xiaozhi Formula on Liver Injury in Diabetic Rats

Figure 3

Effects of Jianpi Xiaozhi Formula (JPXZF) on mRNA and protein expression associated with autophagy reaction in the rat model of type 2 diabetes mellitus (T2DM) with nonalcoholic fatty liver disease (NAFLD) induced by high sugar and high-fat diet combined with streptozotocin (STZ) treatment. Normal control (NC group): healthy untreated rats; diabetes mellitus (DM) group: rat models of DM; DM + JL group: diabetic rats treated with low-dosage Jianpi Xiaozhi formula (JPXZF) (4.5 g/kg/d); DM + JM group: diabetic rats treated with an intermediate dosage of JPXZF (9 g/kg/d); DM + JH group: diabetic rats treated with high-dosage JPXZF (18 g/kg/d); DM + PBA group: diabetic rats treated with 4-phenylbutyric acid (PBA; 2.5 mg/kg/d). (a) The mRNA expression levels of Beclin-1 and P62 were assessed by quantitative real-time polymerase chain reaction; (b, c) Beclin-1, P62, and long-chain 3II/I (LC3II/I) protein expressions were determined by Western blotting (WB). The data are expressed as the means ± standard deviation. versus NC group; versus DM group; versus DM + JL group.

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