Mechanistic Research into the Effects of the Jianpi Xiaozhi Formula on Liver Injury in Diabetic Rats

Figure 4

Effects of Jianpi Xiaozhi formula (JPXZF) on mRNA and protein expression associated with endoplasmic reticulum stress (ERS)-autophagy in rat models of type 2 diabetes mellitus (T2DM) with nonalcoholic fatty liver disease (NAFLD) induced by a high-sugar and high-fat diet combined with streptozotocin (STZ) treatment. Normal control (NC group): healthy untreated rats; diabetes mellitus (DM) group: rat models of DM; DM + JL group: diabetic rats treated with low-dosage Jianpi Xiaozhi formula (JPXZF) (4.5 g/kg/d); DM + JM group: diabetic rats treated with an intermediate dosage of JPXZF (9 g/kg/d); DM + JH group: diabetic rats treated with high-dosage JPXZF (18 g/kg/d); DM + PBA group: diabetic rats treated with 4-phenylbutyric acid (PBA; 2.5 mg/kg/d). (a) The mRNA expression levels of family with sequence similarity 134, member B (FAM134B) were assessed by quantitative real-time polymerase chain reaction. (b, c) FAM134B protein expression was determined by Western blotting (WB). (d) Immunofluorescence image analysis showed the relative expression levels of FAM134B in the rat liver. The nucleus showed blue fluorescence emitted by DAPI. The target protein FAM134B was labelled with green fluorescence. The data are expressed as the means ± standard deviation. versus the NC group; versus the DM group; versus the DM + JL group.

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