Evidence-Based Complementary and Alternative Medicine

Research Article

Antiplasmodial Activity of 80% Methanolic Extract and Solvent Fractions of Stem Bark of Acacia tortilis in Swiss Albino Mice

Table 3

Packed cell volume (PCV), weight, and temperature for the crude extract and solvent fractions of the stem bark of Acacia tortilis in the 4-day suppressive test.


Treatment	PCV			Temperature (°C)			Body weight (g)
Treatment	PCV at D0	PCV at D4	% PCV change	T° at D0	T° at D4	% T° change	Weight at D0	Weight at D4	% Weight change

10 ml/kg DW	54.83 ± 1.40	44.67 ± 1.54	−18.41 ± 2.82	36.73 ± 0.05	34.23 ± 0.24	−6.80 ± 0.70	24.67 ± 1.28	22.00 ± 1.13	−10.78 ± 0.59
CHQ 25 mg/kg	56.50 ± 0.76	55.67 ± 0.67^a3	−1.46 ± 0.29^a3	36.42 ± 0.10	36.43 ± 0.08^a3	0.05 ± 0.19^a3	23.67 ± 1.41	23.50 ± 1.26	−0.56 ±0.56^a3
100 mg/kg CE	54.33 ± 0.67	46.67 ± 1.61^b2	−14.20 ± 2.09^b2	36.40 ± 0.14	34.45 ± 0.12^b3	−5.35 ± 0.59^b3	26.17 ± 1.11	25.17 ± 0.91	−3.65 ± 1.31^a3
200 mg/kg CE	52.83 ± 0.48	47.67 ± 1.61^b2	−9.72 ± 3.23	36.47 ± 0.11	34.97 ± 0.35^b3	−4.12 ± 0.68^a1,b3	24.50 ± 1.52	23.67 ± 1.31	−3.15 ± 1.15^a3
400 mg/kg CE	53.67 ± 0.80	50.83 ± 0.95^a1	−5.17 ± 2.26^a2	36.13 ± 0.09	34.93 ± 0.15^b3	−3.32 ± 0.33^a3,b2	25.00 ± 0.97	24.33 ± 0.84	−2.58 ± 0.82^a3
100 mg/kg AF	55.17 ± 0.83	46.83 ± 1.17^b3	−15.10 ± 1.82^b2	36.35 ± 0.04	34.13 ± 0.08^b3	−6.10 ± 0.22^b3	25.00 ± 0.93	22.67 ± 0.76	−9.14 ± 2.07^b3
200 mg/kg AF	54.17 ± 0.83	46.67 ± 1.31^b3	−13.75 ± 2.67^b2	36.45 ± 0.11	34.48 ± 0.07^b3	−5.39 ± 0.21^b3	25.50 ± 1.38	23.50 ± 1.23	−7.76 ± 0.82^b2
400 mg/kg AF	52.00 ± 0.97	45.17 ± 1.30^b3	−13.01 ± 2.80^b1	36.33 ± 0.06	34.73 ± 0.08^b3,c1	−4.40 ± 0.14^a2,b3	25.17 ± 1.45	23.67 ± 1.05	−5.57 ± 1.45^a1
10 ml/kg 2% T 80	53.17 ± 2.24	44.17 ± 1.22	−16.32 ± 3.57	36.53 ± 0.11	34.08 ± 0.18	−6.71 ± 0.22	26.83 ± 1.01	24.00 ± 0.68	−10.37 ± 1.38
CHQ 25 mg/kg	51.83 ± 2.57	50.67 ± 2.35^a1	−1.60 ± 4.62^a2	36.53 ± 0.09	36.50 ± 0.09^a3	−0.09 ± 0.06^a3	24.83 ± 1.05	25.00 ± 1.15	0.59 ± 0.60^a3
100 mg/kg DF	56.17 ± 2.24	48.33 ± 1.28	−13.53 ± 2.73^a1	36.50 ± 0.09	34.60 ± 0.09^a1,b3,e3	−5.21 ± 0.01^a3,b3,e3	24.67 ± 1.23	23.33 ± 1.12	−5.34 ± 0.70^a3,b2
200 mg/kg DF	53.83 ± 1.14	49.33 ± 1.28	−8.12 ± 3.34^a1	36.58 ± 0.08	35.17 ± 0.09^a3,b3,c1	−3.87 ± 0.09^a3,b3,c3	24.33 ± 1.33	23.50 ± 1.26	−3.37 ± 0.70^a3,b2
400 mg/kg DF	52.17 ± 1.30	48.17 ± 1.19^a1	−5.10 ± 2.56^a2	36.53 ± 0.12	35.50 ± 0.13^a3,b3,c3	−2.83 ± 0.07^a3,b3,d3	24.67 ± 1.26	24.00 ± 1.06	−2.52 ± 0.80^a3
100 mg/kg HF	53.50 ± 1.82	44.50 ± 1.77	−16.89 ± 0.62^b1	36.53 ± 0.09	34.23 ± 0.09^b3	−6.30 ± −6.34^b3,d2	25.50 ± 1.18	22.83 ± 1.05	−10.42 ± 1.09^b3
200 mg/kg HF	54.00 ± 2.39	46.00 ± 2.03	−14.18 ± 4.70	36.50 ± 0.15	34.43 ± 0.16^b3	−5.66 ± −5.91^a1,b3,e2	24.17 ± 1.58	22.00 ± 0.93	−8.24 ± 2.43^b2
400 mg/kg HF	56.67 ± 1.45	49.33 ± 1.36	−12.86 ± 1.89	36.47 ± 0.09	34.67 ± 0.09^a1,b3	−4.94 ± −4.97^a2,b3,c3	25.50 ± 1.18	23.67 ± 0.76	−6.89 ± 1.45^b1

Data are expressed as mean ± SEM; n = 6, a = compared to the negative control, b = compared to the positive control, c = compared to 100 mg/kg, d = compared to 200 mg/kg, e = compared to 400 mg/kg, ¹, ², ³, = negative and positive controls for dichloromethane and hexane fractions, SEM = standard error of the mean, D0 = day 0, D4 = day 4, DW = distilled water, CE = crude extract, AF = aqueous fraction, DF = dichloromethane fraction, HF = hexane fraction, T 80 = tween 80, and CHQ = chloroquine.