[Retracted] Inhibition of miR-29b-1-5p Attenuates Inflammatory Response and Pulmonary Fibrosis in LPS-Induced Acute Lung Injury by Regulating RTN4 Expression

Figure 2

miR-29b-1-5p negatively regulates the RTN4 and Akt/ERK signaling pathway, thus promoting LPS-induced injury and inflammatory response in vitro. (a) Analysis of the miRWalk and RNA22 databases. (b) Luciferase activity assays of the RTN4-Wt and RTN4-Mut in the presence of miR-29b-1-5p mimic or inhibitor. (c), The mRNA level of RTN4 was determined by using qRT-PCR and the protein expression of RTN4A/B, the p-Akt and p-ERK1/2 extent by using Western blot analysis. (d) The levels of IL-1β, IL-8, TNF-α, and IL-6 in the supernatant derived from LPS-conditioned MH-S cells were measured using the ELISA method. (e) The viability of LPS-conditioned MH-S cells was evaluated using CCK-8 assay at indicated time points. (f) The apoptosis rate of LPS-conditioned MH-S cells. , vs. MH-S cells, ^# vs. LPS-conditioned MH-S cells, and vs. MH-S cells treated with miR-29b-1-5p mimic.

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