Liquiritin Alleviates Depression-Like Behavior in CUMS Mice by Inhibiting Oxidative Stress and NLRP3 Inflammasome in Hippocampus

Figure 4

Effects of liquiritin or fluoxetine on the expression of inflammatory proteins and the interactions with proteins. (a) NLRP3 inflammasome-associated protein expression in the hippocampus. β-Actin was used as an internal reference. (b, c) Quantification of NLRP3 and TLR4 protein expression by Western blot (n = 3). (d) Expression of NLRP3 and TLR4 in hippocampal CA3 region measured by IHC. Positive cells are represented as brown spots. (e, f) Quantification of NLRP3 and TLR4 expression by IHC (n = 3). (g, h) CETSA and quantification. Quantification of both Western blot and IHC was performed using ImageJ software. Data represent means ± SD (n = 3). For statistical significance, , , and versus control group; and versus the CUMS group.

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