Anaphylactic Rare Saponins Separated from Panax notoginseng Saponin and a Proteomic Approach to Their Anaphylactic Mechanism

Figure 1

Anaphylactic property test results of each subfraction. The anaphylactic property was tested by the observation of RBL-2H3 cells incubated with each subfraction using an inverted microscope (200x). RBL-2H3 cells showed no degranulation in (a) the negative control group and strong degranulation (blurred cell membrane and obvious granules) in (b) the positive control group. Cells in the group of (d) Fr. 3, (f) Fr. 5, and (h) Fr. 7-8 showed anaphylactoid reactions (>50% degranulated cells). Cells in the group of (c) Fr. 1-2, (e) Fr. 4, (g) Fr. 6, (i) Fr. 9-10, and (j) Fr. 11 showed no anaphylactoid reactions (<30% degranulated cells).

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)