Anaphylactic Rare Saponins Separated from Panax notoginseng Saponin and a Proteomic Approach to Their Anaphylactic Mechanism

Figure 2

β-HexRRs of all tested groups and the anaphylactic ability of each saponin. β-HexRRs of the control groups, C 48/80 groups, noto-ST-4 groups, Rg3 groups, Rh4 groups, Rk3 groups, gyp-LXXV groups, and noto-T5 groups were tested with different incubation times of (a) 15 min; (b) 30 min; (c) 1 h; and (d) 2 h. (e) Anaphylactic ability of each saponin at a concentration of 0.03 μmol/mL was determined by the β-HexRRs of the control groups and C 48/80 groups. L = low dose group, M = medium dose group, and H = high dose group. The concentrations of each low dose group, medium dose group, and high dose group are shown in Table 2. Each group was repeated 3 times, and the data are expressed as the mean ± SEM (n = 3). and , compared with the corresponding control group (t-test). The β-HexRR of the high dose group of Rh4 with an incubation time of 1 h was significantly lower than that of the control group, while all the other significant differences were significantly higher.

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