Solamargine Alleviates Proliferation and Metastasis of Cervical Cancer Cells by Blocking the CXCL3-Mediated Erk Signaling Pathway

Figure 2

The effect of solamargine on the metastasis of cervical cancer cells and its potential mechanism related to the Erk pathway. (a) Scratch test was performed to assess the migration of tumor cells under 0, 5, and 10 μM solamargine treatment after 24 h (×100 magnification, scale bar = 100 μm). (b) Transwell assay was used to measure the invasion of solamargine-treated cells (×250 magnification, scale bar = 50 μm). (c) Following exposure to solamargine, western blot was conducted to detect p-Erk1/2 and Erk1/2 protein levels. GAPDH served as the loading control. (d, e) qRT-PCR was performed to detect the expression of PBK, RACK1, CXCL3, TRIP4, and SEMA3C in solamargine-treated cells. GAPDH served as the internal control. (f, g) Western blot was conducted to detect the protein expression of CXCL3 in solamargine-treated cells. , , and vs. 0 μM. h, hours; Erk, extracellular regulated protein kinases; p, phosphorylated; qRT-PCR, quantitative real-time polymerase chain reaction; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; PBK, PDZ binding kinase; RACK1, receptor for activated C kinase 1; CXCL3, C-X-C motif chemokine ligand 3; TRIP4, thyroid hormone receptor interactor 4; SEMA3C, semaphorin 3C.

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