Astragaloside IV Improves the Barrier Damage in Diabetic Glomerular Endothelial Cells Stimulated by High Glucose and High Insulin

Figure 2

Cell glucose uptake ability and ROS content assay under high insulin + glucose condition. (A) High insulin concentration-dependently inhibited cell glucose uptake. (a)–(d) The concentration of insulin at 0, 0.1, 1, and 10 μM. (B) 2-NBDG fluorescence analyzed for data (A). (C) High glucose concentration-dependently inhibited cell glucose uptake. (a)–(d) The concentration of insulin at 0, 15, 30, and 60 mM. (D) 2-NBDG fluorescence analyzed for data (C). (e) High insulin concentration-dependently promoted cell oxidative stress. (a)–(d) The concentration of insulin at 0, 0.1, 1, and 10 μM. (f) 2-NBDG fluorescence analyzed for data (e). (g) High glucose concentration-dependently promoted cell oxidative stress. (a)–(d) The concentration of insulin at 0, 15, 30, 60 mM. (H) 2-NBDG fluorescence was analyzed for data (g);  < 0.05,  < 0.01,  < 0.01 vs. control.

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