Astragaloside IV Improves the Barrier Damage in Diabetic Glomerular Endothelial Cells Stimulated by High Glucose and High Insulin

Figure 3

Effects of astragaloside IV(AS-IV) on barrier integrity of GEnCs cells stimulated by high insulin and high glucose. (a) The assay of transendothelial electrical resistance (TEER). In a normal environment, the maximum TEER value in GEnCs was within days 15–21;  < 0.001 vs.7 d; ^### < 0.001 vs.9 d; ^&&& < 0.001 vs.11 d; ^% < 0.05, ^%% < 0.01 vs. 13 d. (b) After high glucose and high insulin stimulation, dextran in the lower was significantly increased,  < 0.001 vs. control. (c) After drug stimulation, the TEER value was significantly reduced;  < 0.05,  < 0.01,  < 0.001 vs. control; ^# < 0.05, ^## < 0.01, ^### < 0.001 vs. H-Glu + Ins. (d) AS-IV treatment reduced leakage of dextran (70 kD) from the monocellular barrier,  < 0.001 vs. control; ^### < 0.001 vs. H-Glu + Ins.

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