Astragaloside IV Improves the Barrier Damage in Diabetic Glomerular Endothelial Cells Stimulated by High Glucose and High Insulin

Figure 4

Effects of astragaloside IV on tight junction proteins in GEnCs cells. Expressions of (a) ZO-1 and (d) claudin-5 were determined by immunofluorescence assay under the laser scanning confocal microscope. Relative fluorescence intensities of (b) ZO-1 and (e) claudin-5 were determined by Image-J software. WB detection for proteins expression and quantitative analysis of ZO-1 (c) and claudin-5 (f);  < 0.05,  < 0.01,  < 0.001 vs. H-Glu + Ins.

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