Astragaloside IV Improves the Barrier Damage in Diabetic Glomerular Endothelial Cells Stimulated by High Glucose and High Insulin

Figure 5

Akt/GSK3α/β signaling pathway evaluation. (a) Expression of p-Akt, Akt, p-GSK3α/β, GSK3α/β, and GAPDH determined by Western blot. Relative expression of the proteins was assayed by Image-J software, and ratio of (b) p-Akt/Akt, (c) p-GSK3α/GSK3α, and (d) p-GSK3β/GSK3β was obtained;  < 0.05,  < 0.01,  < 0.001 vs. H-Glu + Ins.

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