Downregulation of Circ-PITHD1 Suppressed Colorectal Cancer via Glycolysis Inhibition through miR-590-5p/HK2 Axis

Figure 2

Downregulation circ-PITHD1 suppressed CRC proliferation and tumor growth in both in vivo and in vitro experiments. (a) RT-qPCR detection shows the expression of circ-PITHD1 in both Lovo and SW480. The data are presented as the mean ± SD. vs. si-NC. (b, c) CCK8 detection shows the effect of circ-PITHD1 on CRC cell proliferation. The data are presented as the mean ± SD. vs. si-NC. (d, e) Cloning formation assay showing the cell proliferation in both Lovo and SW480 cells. Data are presented as the mean ± SD. vs. si-NC. (f) Representative photographs of SW480 tumor formation in xenografts of nude mice. (g, h) Summary of tumor volumes and weight in mice. Data are presented as the mean ± SD. vs. sh-NC. (i, j) Immunohistochemistry shows the percentage of Ki-67-positive cells. The relative Ki-67-positive cells were calculated. Data are presented as the mean ± SD. vs. sh-NC.

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