Downregulation of Circ-PITHD1 Suppressed Colorectal Cancer via Glycolysis Inhibition through miR-590-5p/HK2 Axis

Figure 4

Both miR-590-5p and HK2 were the downstream targets of circ-PITHD1. (a) The luciferase activity of circ-PITHD1 in HEK293T cells transfected with different miRNA mimics, which are putative binding sites for the circ-PITHD1 sequence. Luciferase activity was normalized by Renilla luciferase activity. (b) Prediction of binding sites of miR-590-5p in circ-PITHD1. The MUT version of circ-PITHD1 is presented. (c) Relative luciferase activity determined 48 h after transfection of HEK293T cells with miR-590-5p mimic/NC or circ-PITHD1 WT/Mut. Data are presented as means ± SD. . (d) Prediction of binding sites of miR-590-5p within the 3′UTR of HK2. The MUT version of 3′-UTR-HK2 is shown. (e) Relative luciferase activity determined 48 h after transfection of HEK293T cells with miR-590-5p mimic/NC or 3′UTR-HK2 WT/Mut. Data are presented as means ± SD. . (f–k) RT-qPCR detection shows the expression of circ-PITHD1, miR-590-5p, and HK2 in both Lovo and SW480 after being transfected with si-circ-PITHD1, miR-590-5p inhibitor, and HK2 overexpression vector single or combined. Data are presented as means ± SD. vs. NC. ^### vs. si-circ-PITHD1.

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