[Retracted] TGF-β1 Induces Interlukin-11 Expression and Pro-Fibrotic Effect by DNA Demethylation in Subconjunctival Fibroblasts

Figure 2

TGF-β1 and DNMT inhibitor can up-regulate the mRNA and protein expressions of IL11 in the HTFs. (a) The HTFs were treated with 12 h serum starvation followed by 10 ng/mL TGF-β1 for 0, 12, and 24 h; RT-qPCR was utilized to determine the expression change of IL11. (b) ELISA tests of IL11 protein expression in the supernatant of the HTFs after TGF-β1 treatment. (c) The HTFs were serum-starved for 12 h and then treated by 5-Aza-dc (5 μM), TGF-β1 (10 ng/mL), and TGF-β1 together with 5-Aza-dc for 24 h. A DMEM-alone group was included as a control. RT-qPCR was adopted for assessing the changes in the expressions of IL11. (d) ELISA tests of IL11 protein expression in the supernatant of the HTFs of the corresponding groups. Data were SD and mean from three independent examinations, each with triplicate samples. , vs. 0 h value. ^##, ^### vs. control group at the same time point by one-way ANOVA followed by the Fisher test (T represents TGF-β1; 5A represents 5-Aza-dc).

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