Esculentoside A Alleviates Intestinal Dysmotility in Ulcerative Colitis by Regulating H₂S/CSE and NO/nNOS Systems

Figure 2

Effects of Esculentoside A on hydrogen sulfide/cystathionine γ-lyase and NO/neuronal nitric oxide synthase systems in the dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) rat model. (a) The contents of hydrogen sulfide (H2S) and NO in serum samples of the sham, model, Esculentoside A (EsA)-10, and EsA-20 groups. (b) The expression of cystathionine β-synthase (CBS), cystathionine γ-lyase (CSE), and neuronal nitric oxide synthase (nNOS) in the sham, model, EsA-10, and EsA-20 groups by immunohistochemistry. (c, d) The mRNA and protein levels of CSE, CBS, and nNOS in the sham, model, EsA-10, and EsA-20 groups by quantitative reverse transcription polymerase chain reaction and western blotting. , , and vs. model by one-way ANOVA followed by Tukey’s post hoc test (n = 3).

(a)

(b)

(c)

(d)