Esculentoside A Alleviates Intestinal Dysmotility in Ulcerative Colitis by Regulating H₂S/CSE and NO/nNOS Systems

Figure 3

Effects of Esculentoside A on LPS-induced intestinal neuronal cells. (a) The cell morphology of primary intestinal neuronal cells. (b) The expression of neuron-specific enolase (NSE) in primary intestinal neuronal cells by cell immunofluorescence. (c–e) Cell viability, proliferation, and apoptosis of primary intestinal neuronal cells in control, lipopolysaccharide (LPS), Esculentoside A (EsA)-10, and EsA-20 groups assessed using the CCK-8 assay, 5-Ethynyl-2′-deoxyuridine (EdU) assay, and flow cytometry. (f) The contents of interleukin 6 (IL-6) and tumor necrosis factor-α (TNF-α) in the primary intestinal neuronal cells of control, LPS, EsA-10, and EsA-20 groups using enzyme-linked immunosorbent assay. (g) The mRNA and protein levels of cystathionine γ-lyase, cystathionine β-synthase, and neuronal nitric oxide synthase in primary intestinal neuronal cells of the control, LPS, EsA-10, and EsA-20 groups using quantitative reverse transcription polymerase chain reaction and western blotting. , , , and vs. LPS by one-way ANOVA followed by Tukey’s post hoc test (n = 3).

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