Verbena Attenuates Adriamycin-Induced Renal Tubular Injury via Inhibition of ROS-ERK1/2-NLRP3 Signal Pathway

Figure 4

TAEV ameliorates ADR-induced oxidative stress injury in the cells. (a) Cells were insulted with ADR (1 μg/mL) for several times (0, 2, and 4 h and then incubated with DHE agent for another 40 min (bar = 100 μm). (b) Cells pretreated with TAEV (1 mg/mL) and NAC (10 μM) for 1 h were challenged with ADR and then detected using a ROS detection kit. (c) Cells pretreated with TAEV (1 mg/mL) and NAC (10 μM) for 1 h were stimulated with ADR (1 μg/mL) for another 4 h. Then, cellular lysates were analyzed using a OxyBlot protein oxidation detection kit. (^# versus control and versus ADR).

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