Verbena Attenuates Adriamycin-Induced Renal Tubular Injury via Inhibition of ROS-ERK1/2-NLRP3 Signal Pathway

Figure 6

TAEV inhibited ADR-induced ERK1/2-mediated activation of NLRP3 signal pathway. (a) Knocking down ERK1/2 with specific siRNA (). (b) Cells were silenced by ERK1/2 specific siRNA or pretreated with U0126 (20 μM). Then, cells were challenged with ADR (1 μg/mL) for another 9 h (^## versus control; and versus ADR). (c) Cells were silenced by ERK1/2 specific siRNA or pretreated with TAEV (1 mg/mL) and then insulted with ADR (1 μg/mL) for another 9 h (^# versus control and versus ADR). (d) Cells were pretreated with TAEV (1 mg/mL) and INF39 (100 μM) for 1 h and then stimulated with ADR (1 μg/mL) for another 12 h (^## versus control and , , and versus ADR).

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