Research Article

Overexpression of SRD5A3 in Hepatocellular Carcinoma and Its Molecular Mechanism: A Study of Bioinformatics Exploration Analysis with Experimental Verification

Figure 6

The experimental validation of SRD5A3 in HCC cell lines. (a) SRD5A gene expression showed a correlation with the expression of genes shown below: SRD5A1, HSD17B6, HSD17B3, HSD17B2, DOLK, CYP17A1, AKR1D1, AKR1C3, AKR1C2, and AKR1C4. (b) The knockdown efficiency of SRD5A3 through two siRNA was confirmed by RT-PCR. (c) RT-PCR revealed that the level of DOLK was consistently significantly increased by the SRD5A3 loss among five genes. ; ; , , NS, no significance.
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