Zhuye Shigao Decoction Combined with Qingqi Huatan Pills in Alleviating the Acute Exacerbation of Chronic Obstructive Pulmonary Disease (Phlegm-Heat Stagnating in the Lungs) via the IL-6-Mediated JAK1/STAT3 Signaling Pathway

Figure 4

Expression of the JAK1/STAT3 signaling pathway of lung tissue in each group. (a) JAK1 protein expression of lung tissue detected by immunohistochemistry is presented by photomicrographs. The nucleus is colored in blue and the positive cells are colored in brown (scale bar is 50 µm, ×400). (b) The strip chart of JAK1 and STAT3, β-actin was used as the internal reference. (c) The relative JAK1 gene expression. (d) Semiquantitative analysis based on the intensity of strip chart for JAK1 in lung tissue. (e) STAT3 protein expression of lung tissue detected by immunohistochemistry was presented by photomicrographs. The nucleus is colored in blue and the positive cells are colored in brown (scale bar is 50 µm, ×400). (f) Semiquantitative analysis of STAT3 expression. (g) Gene expression of STAT3. Error bars represent SD, n = 3. , ^#, ^&, and ^Δ represent values <0.05 which are considered statistically significant. versus MC (model control), ^# versus herbs low, ^& versus herbs mid, and ^Δ versus herbs high.

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